Journal: Cancer Cell International

Article Title: Methotrexate-triggered ferroptosis suppresses oral cancer progression by phosphorylated KEAP1-mediated NRF2 degradation to inhibit SLC7A11/GPX4 signaling pathway

doi: 10.1186/s12935-025-04019-y

Figure Lengend Snippet: MTX significantly inhibits the activities of OC cells. A Chemical formula of methotrexate (MTX). B-C A253 and MOC1 cells were treated with various MTX concentrations for different periods, and cell viability was measured using the CCK-8 assay ( n = 3). D-E Representative flow cytometry of cell apoptosis assays in OC cells, including A253 cells ( D ) and MOC1 cells ( E ), after treatment with various concentrations of MTX for 24 h, and the quantitative data are presented ( n = 3). F Representative flow cytometry of PI staining in A253 cells after treatment with various concentrations of MTX for 24 h, and the quantitative data are presented ( n = 3). G-H Representative results of colony formation assays in OC cells, including A253 cells ( G ) and MOC1 cells ( H ), at 14 days after treatment with various concentrations of MTX, and the quantitative data are presented ( n = 3). I-J Representative results of wound-healing assays in OC cells, including A253 cells ( I ) and MOC1 cells ( J ), at 0, 24, and 48 h after treatment with various concentrations of MTX ( n = 3)

Article Snippet: Methotrexate (MTX) was purchased from MedChemExpress (HY-14519) (MCE, Shanghai, China), and was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution of 10 mM and stored in −20°C for usage.

Techniques: CCK-8 Assay, Flow Cytometry, Staining